试题与答案

农业科技工作者在烟草中找到了一种抗病基因,现拟采用基因工程技术将该基因转入棉花,

题型:读图填空题

题目:

农业科技工作者在烟草中找到了一种抗病基因,现拟采用基因工程技术将该基因转入棉花,培育抗病棉花品系。请完成下列问题。

(1)为了能把该抗病基因转入到棉花细胞中,常用的载体是___________。

(2)要使载体与抗病基因连接,首先应使用________对其进行切割。假如载体被切割后,得到的分子末端序列为则能与该载体连接的抗病基因分子末端是_________。

(3)切割完成后,采用______将载体与该抗病基因连接,连接后得到的DNA分子称为_______。

(4)再将连接得到的DNA分子导入农杆菌,然后用该农杆菌去____棉花细胞,利用植物细胞具有的____性进行组织培养,从培养出的植株中_______出抗病的棉花。

(5)该抗病基因在棉花细胞中表达的产物是_______。转基因棉花获得的______是由该表达产物来体现的。

答案:

(1)Ti质粒

(2)限制性核酸内切酶(限制酶)    A

(3)DNA连接酶   重组DNA

(4)感染   全能   筛选(选择)

(5)蛋白质    抗病性状

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