试题与答案

在培育转基因植物的研究中,卡那霉素抗性基因(kan)常作为标记基因,只有含卡那霉

题型:读图填空题

题目:

在培育转基因植物的研究中,卡那霉素抗性基因(kan)常作为标记基因,只有含卡那霉素抗性基因的细胞才能在卡那霉素培养基上生长。下图为获得抗虫棉的技术流程。请据图回答:

(1)A过程需要的酶有________________。

(2)B过程及其结果体现了质粒作为运载体必须具备的两个条件是________、_______。

(3)C过程的培养基除含有必要营养物质、琼脂和激素外,还必须加入________________。

(4)如果利用DNA分子杂交原理对再生植株进行检测,D过程应该用____________________作为探针。

(5)科学家发现转基因植株的卡那霉素抗性基因的传递符合孟德尔遗传规律。

①.将转基因植株与______杂交,其后代中抗卡那霉素型与卡那霉素敏感型的数量比为1:1。

②.若该转基因植株自交,则其后代中抗卡那霉素型与卡那霉素敏感型的数量比为________。

③.若将该转基因植株的花药在卡那霉素培养基上作离体培养,则获得的再生植株群体中抗卡那霉素型植株占_________%。

答案:

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答案:A

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